| Autor: |
Cvejić, Jelena H., Rohmer, Michel |
| Zdroj: |
Phytochemistry; January 2000, Vol. 53 Issue: 1 p21-28, 8p |
| Abstrakt: |
Isoprenoid biosynthesis was investigated in the two diatoms Phaeodactylum tricornutumand Nitzschia ovalisby labeling experiments performed in mixotrophic growth conditions with sodium [1-13C]acetate, 13CO2, [1-13C]glucose, sodium [3-13C]pyruvate and 1-deoxy-D-[5,5-2H2]xylulose. A clear dichotomy was found. Acetate was the preferred carbon source for the formation of the sterols in the cytoplasm via the mevalonate pathway. Carbon dioxide was the main source for phytol biosynthesis in the chloroplasts via the mevalonate-independent methylerythritol 4-phosphate pathway. The two diatoms showed the same compartmentation for isoprenoid biosynthesis as that previously found in higher plants, the red alga Porphyridium cruentumand the Chrysophyte Ochromonas danica. |
| Databáze: |
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| Externí odkaz: |
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