The use of real-time PCR in the diagnosis and monitoring of Mycoplasma haemofeliscopy number in a naturally infected cat

Autor: Braddock, J.A., Tasker, S., Malik, R.
Zdroj: Journal of Feline Medicine & Surgery; June 2004, Vol. 6 Issue: 3 p161-165, 5p
Abstrakt: A 5-year-old male neutered cat was diagnosed with severe anaemia due to acute Mycoplasma haemofelisinfection. Inflammatory respiratory disease was present concurrently. The cat was treated successfully using a fresh transfusion of whole blood and a 6 week course of doxycycline. The patient made a rapid recovery although the allergic airway disease subsequently required specific therapy consisting of inhaled fluticasone and salbutamol. Real-time quantitative PCR assays confirmed the presence of M. haemofelisDNA copies in the blood at presentation. Repeat PCR assays showed a reduction in copy number during treatment and negative PCR results were obtained both 91 and 425 days after presentation. The report describes, for the first time, the use of real-time PCR in the diagnosis and monitoring of natural M. haemofeliscopy number, as well as the induction of long-term negative PCR status.
Databáze: Supplemental Index