| Autor: |
Spicer, Douglas, Astle, Thomas, Booth, Lisa, House, Russell, Hughes, Karin, Kaiser, Robert, Springer, Amy |
| Zdroj: |
Journal of Laboratory Automation; August 2002, Vol. 7 Issue: 4 p50-57, 8p |
| Abstrakt: |
We have developed a method for the automated purification of DNA sequencing reactions using the RapXtract(tm) 384 Dye Terminator Removal Kit and the Quadra 3(tm) Workstation. The process enables purification of 384 reactions in five minutes, significantly impacting the throughput potential of sequencing laboratories. The RapXtract technology utilizes superparamagnetic particles, (i.e., particles that are not themselves magnetic but that respond to a magnetic field) and eliminates the need for centrifugation, vacuum filtration, or modified primers. The Quadra 3 Workstation is a 384-channel liquid handling system, fitted with a retractable magnetic nest designed to incorporate a 384 magnetic separator. The combined technologies result in reduced variability associated with manual methods for sequencing reaction purification. |
| Databáze: |
Supplemental Index |
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