Anticonvulsant Activity of Novel Derivatives of 2- and 3-Piperidinecarboxylic Acid in Mice and Rats

Autor: HINKO, C.N., CRIDER, A.M., KLIEM, M.A., STEINMILLER, C.L., SEO, T.H., HO, BIN, VENKATARANGAN, P., EL-ASSADI, A.A., CHANG, HYEJUNG, BURNS, C.M., TIETZ, E.I., ANDERSEN, P.H., KLITGAARD, H.
Zdroj: Neuropharmacology; December 1996, Vol. 35 Issue: 12 p1721-1735, 15p
Abstrakt: The relative ability of derivatives of 2-piperidinecarboxylic acid (2-PC; pipecolic acid) and 3-piperidinecarboxylic acid (3-PC; nipecotic acid) to block maximal electroshock (MES)-induced seizures, elevate the threshold for electroshock-induced seizures and be neurotoxic in mice was investigated. Protective index (PI) values, based on the MES test and rotorod performance, ranged from 1.3 to 4.5 for 2-PC benzylamides and from <1 to >7.2 for 3-PC derivatives. PI values based on elevation of threshold for electroshock-induced seizures and rotorod performance ranged from >1.6 to >20 for both types of derivatives. Since preliminary data indicated that benzylamide derivatives of 2-PC displace [3H]1-[1-(2-thienyl)-cyclohexyl]piperidine (TCP) binding to the phencyclidine (PCP) site of the N-methyl-d-aspartate (NMDA) receptor in the micromolar range and such low affinity uncompetitive antagonists of the NMDA receptor-associated ionophore have been shown to be effective anticonvulsants with low neurological toxicity, the 2-PC derivatives were evaluated in rat brain homogenates for binding affinity to the PCP site. Although all compounds inhibited [3H]TCP binding, a clear correlation between pharmacological activity and binding affinity was not apparent. Select compounds demonstrated minimal ability to protect against pentylene-tetrazol-,4-aminopyridine- and NMDA-induced seizures in mice. Corneal and amygdala kindled rats exhibited different sensitivities to both valproic acid and the nonsubstituted 2-PC benzylamide, suggesting a difference in these two models. Enantiomers of the α-methyl substituted benzylamide of 2-PC showed some ability to reduce seizure severity in amygdala kindled rats. © 1997 Elsevier Science Ltd. All rights reserved.
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