| Autor: |
van den Akker, Eric, Lutgerink, Jan T., Lafleur, M.Vincent M., Joenje, Hans, Retèl, Jan |
| Zdroj: |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis; August 1994, Vol. 309 Issue: 1 p45-52, 8p |
| Abstrakt: |
Single-stranded M13mp10 DNA containing a 144-bp mutational target sequence in the lacZα gene was treated with singlet (1O2) generated by thermodissociation of the endoperoxide of 3,3′-(1,4-naphthalene-1,4-diyl)diproportionate (NDPO2). After transfection to non-SOS-induced E. colicells, 32 mutants preselected for a mutation in the 144-bp target were collected and analyzed by DNA sequencing. One-G deletions represented the predominant type of mutation accounting for 50% of the mutations analyzed. The remaining part appeared to consist of base substitutions, i.e. G → T transversions (34%), C → T transitions (12.5%) and one T → C transition (3%). Sixty percent of the mutations were found in two major mutational hotspots. We conclude that the predominant one-G deletions are due to a guanine reaction product which might be specific for 1O2. |
| Databáze: |
Supplemental Index |
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