| Abstrakt: |
Metabolism of [14C] gibberellin(GA)12-aldehyde and [14C]GA12in vegetative tissue of potato (Solanum tuberosumssp. andigena) was studied by supplying solutions of these compounds to the base of isolated shoot tips and analyzing the metabolic products by radiodetection after HPLC. Endogenous gibberellins that co-eluted with the 14C-metabolites on HPLC were identified by GC-MS as GA53-aldehyde, GA53, GA44, GA19, GA20, GA29, GA1, and GA8. Thus the early-13-hydroxylation pathway seems to be a major pathway in vegetative potato shoots. This was confirmed by time-course feeding studies; according to HPLC elution profiles, the members of the pathway were produced from [14C]GA12-aldehyde and [14C]GA12in the expected time sequence. The formation of GA53appeared to be predominantly via GA12rather than GA53-aldehyde. The detection of endogenous GA51suggests that the non-hydroxylation pathway was also active. Comparisons of HPLC retention times of radioactive metabolites before base hydrolysis with retention times after base hydrolysis showed that potato shoots can conjugate several GAs. HPLC retention times of other radioactive metabolites corresponded with previously published retention times of GA29-catabolite, GA51-catabolite, GA12-aldehyde-conjugate, and GA53-aldehyde-conjugate. |
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