| Autor: |
Abe, Toshinori, Kudo, Masumi, Oka, Yasuhiro, Yamaguchi, Junji, Sasahara, Takeo |
| Zdroj: |
Journal of Plant Physiology; January 1996, Vol. 149 Issue: 5 p592-598, 7p |
| Abstrakt: |
Changes in α-amylase activity in rice calli during organogenesis were investigated in 5 rice varieties that exhibit different abilities for plant regeneration. During organ differentiation in rice callus tissues, samples were taken at 5-day intervals up to 35 days and α-amylase (EC. 3.2.1.1) activities were measured. The activity in regenerative calli began to increase 15 to 20 days after transfer to the regeneration medium and were elevated 7–11 fold during the culture period, while the calli transferred to callus maintenance medium did not increase and maintained a stable state. The increase in α-amylase activity in regenerative calli was more rapid in the calli that showed higher regenerative abilities (Sasanishiki, Tadukan and Tetep) than in the calli that showed lower regenerative abilities (Fujisaka 5 and Nipponbare). Correlation coefficients between frequencies of organ differentiation and α-amylase activity in the calli 35 days after transfer to the regeneration medium was highly significant. Expression of a rice α-amylase gene (RAmylA) in callus tissues during organ differentiation was examined by Northern blot and Western blot analyses. Messenger RNA from the RAmylAgene, which is a main transcript in germinating rice seeds, was highly expressed in the regenerating calli 15 days after transfer. The translated product from the mRNA of the RAmylA gene and α-amylase isozyme band I (1A and 1B) were highly expressed 20 days after transfer, especially in the regeneration medium. |
| Databáze: |
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