Effect of Macerozyme on Secondary Metabolism Plant Product Production and Phospholipase C Activity in Catharanthus roseusHairy Roots

Autor: Moreno-Valenzuela, Oscar A., Monforte-González, Miriam, Armando Muñoz-Sánchez, J., Méndez-Zeel, Marcela, Loyola-Vargas, Victor M., Teresa Hernández-Sotomayor, S.M.
Zdroj: Journal of Plant Physiology; October 1999, Vol. 155 Issue: 4-5 p447-452, 6p
Abstrakt: The effect of macerozyme on accumulation of indole alkaloid and coumarine and on the induction of tryptophan decarboxylase (TDC, E.C. 4.1.1.28), phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5) and phosphatydilinositol 4,5-bisphosphate phospholipase C (PLC, E.C. 3.1.4.3) activities on Catharanthus roseushairy roots was investigated. Increasing concentrations of macerozyme induced an increase in indole alkaloid and coumarine accumulation. There was a 10-fold increase in TDC and a 4-fold increase in PAL activities in hairy roots treated with 1 % of macerozyme. In a dose-response experiment PLC activity decreased 38 % with 0.5 % of macerozyme and increased 40 % with 1 % of macerozyme when compared to untreated roots. The results demonstrate that macerozyme treatment can affect the production of secondary metabolites and PLC activity in C. roseushairy roots.
Databáze: Supplemental Index