| Autor: |
Szekeres, G., Le Tourneau, A., Benfares, J., Audouin, J., Diebold, J. |
| Zdroj: |
Pathology - Research and Practice; February 1995, Vol. 191 Issue: 1 p52-56, 5p |
| Abstrakt: |
Immunostaining of the cell cycle-associated Ki-67 antigen was studied on routinely formalin-fixed and paraffin-embedded tissue sections, using the Ki-67-specific monoclonal antibody MIB-1. Immunomorphological analysis of the Ki-67 immunostaining pattern was carried out following tissue pre-treatments including combinations of microwave heating and trypsinization, as well as of ribonuclease and deoxyribonuclease pre-digestion of the sections. The nucleolar Ki-67 immunostaining after slide pre-treatment by microwave heating followed by trypsinization was reduced only by ribonuclease pre-digestion, if this latter was used before heating. Ki-67 immunostaining was not significantly reduced by deoxyribonuclease treatment. We conclude that Ki-67, located in the nucleoli, may be associated there with nucleolar RNA. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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