| Autor: |
Berchtold, Manfred, König, Helmut |
| Zdroj: |
Systematic and Applied Microbiology; March 1996, Vol. 19 Issue: 1 p66-73, 8p |
| Abstrakt: |
Different small subunit ribosomal DNA (SSU rDNA) sequences of uncultivated spirochetes from the hindgut of the termite Mastotermes darwiniensiswere determined and phylogenetically analysed. Starting with DNA isolated from hindgut contents we amplified SSU rDNA coding regions by PCR using bacteria- and spirochete-specific primers, respectively. Cloning and sequencing of the amplification products re-sulted in 11 different spirochetal SSU rDNA sequences with similarity values of 81.8% to 99.0% to each other. Phylogenetic trees showed that the obtained sequences are related to the genus Treponemawith 84.0% to 91.1% similarity to Treponemaspec. H1 and Spirochaeta stenostreptaas their nearest relatives among the cultivated spirochetes. Using fluorescence in situhybridisation one of the SSU rDNA sequences could be assigned to a large spirochete. So at least one of the large hindgut spirochetes unique in termite hindguts and assigned to the genera Hollandina, Pillotina, Clevelandinaor Diplocalyxphylogenetically belongs to the treponeme cluster of spirochetes. |
| Databáze: |
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