| Autor: |
Perreten, Vincent, Kollöffel, Beat, Teuber, Michael |
| Zdroj: |
Systematic and Applied Microbiology; January 1997, Vol. 20 Issue: 1 p27-38, 12p |
| Abstrakt: |
A Tn916-like transposon (TnFO1) was found in the multiple antibiotic resistant Enterococcus faecalisstrain FO1 isolated from a raw milk cheese. In this strain, the tetracycline determinant was localized by DNA-DNA hybridization with a tetMnucleotide probe on the chromosome and on a 30-kb plasmid. The transposon TnFO1was identified and characterized by DNA-DNA hybridization experiments with the five internal HincII fragments of Tn916. The tetracycline resistance determinant was identified by its complete nucleotide sequence as TetM. Transposon TnFO1was also detected in its circular form by DNA-DNA hybridization and PCR amplification. Both ends including the joining region of the closed circular transposon TnFO1were sequenced. TnFO1could be transferred by conjugation from Enterococcus faecalisinto Enterococcus faecalis, Lactococcus lactissubsp. lactisbiovar. diacetylactis, Listeria innocua, Leuconostoc mesenteroidesand Staphylococcus aureus, and from Lactococcus lactissubsp. lactisbiovar. diacetylactisinto Listeria innocua. Pulsed-field electrophoresis of genomic DNA from E. faecalisFO1 transconjugants showed that transposon TnFO1integrated at different sites. |
| Databáze: |
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