| Autor: |
Høie, Steiner, Dalsgaard, Inger, Aase, Inger Lise, Heum, Marianne, Thornton, Jacinta M., Powell, Richard |
| Zdroj: |
Systematic and Applied Microbiology; September 1999, Vol. 22 Issue: 3 p403-411, 9p |
| Abstrakt: |
Two hundred and five isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and countries were characterized by polymerase chain reaction (PCR) targeting four genes. The chosen genes were those encoding the extracellular A-layer protein (AP), the serine protease (Sprot), the glycerophospholipid:cholestrol acetyltransferase protein (GCAT), and the 16S rRNA (16S rDNA). All the atypical A. salmonicidaisolates could be assigned to 4 PCR groups. Group 1 comprised 45 strains which tested positive for PCR amplification, using the 16S rDNA, GCAT2, Sprot2, and AP primer-sets. Group 2 comprised 88 strains with produced PCR products using the 16S rDNA, GCAT2 and AP primer-sets. Group 3 comprised 21 strains which produced PCR products using 16S rDNA, GCAT2 and Sprot2 primer-sets, and group 4 comprised 51 strains which produced PCR products using the 16S rDNA and GCAT2 primer-sets only. A. salmonicidasubsp. salmonicidaisolates tested, belonged to group 1. The PCR primer-sets separated A. salmonicidafrom other reference strains of Aeromonasspecies and related bacteria with the exception of Aeromonas hydrophila. The results indicated that PCR typing is a useful framework for characterization of the increasing number of isolations of atypical A. salmonicida. |
| Databáze: |
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