Inhibitory effects of N,N′-diphenyl-p-phenylenediamine on the early stage of the enhanced hepatocarcinogenesis caused by coadministration of ethionine and a choline-deficient L-amino acid-defined diet in rats

Autor: Kobayashi, Eisaku, Tsujiuchi, Toshifumi, Nakae, Dai, Mizumoto, Yasushi, Andoh, Nobuaki, Endoh, Takehiro, Kitada, Hiromichi, Tsutsumi, Masahiro, Denda, Ayumi, Konishi, Yoichi
Zdroj: Experimental and Toxicologic Pathology; June 1996, Vol. 48 Issue: 4 p275-282, 8p
Abstrakt: Effects of N,N′-diphenyl-p-phenylenediamine (DPPD), an antioxidant, on liver carcinogenesis caused by a choline-deficient L-amino acid-defined (CDAA) diet containing ethionine were studied in Fischer 344 rats. Male animals, 6 weeks old, were fed a CDAA diet, a choline-supplemented L-amino acid-defined (CSAA) diet or a CDAA diet containing 0.05 % ethionine with or without 0.2 % DPPD. Histological changes and lesions positive for γ-glutamyltransferase (GGT) were analyzed 12 weeks after the beginning of the experiment. The levels of 8-hydroxyguanine (8-OHGua) in DNA and 2-thiobarbituric acid-reacting substances (TBARS) were measured as the parameters for cellular oxidative damage after 4 and 11 days of treatment. Expression of c-mycand c-Ha-raswas also investigated in relation to cell proliferation after 2, 4, 8 and 11 days.
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