| Autor: |
Ali-Vehmas, Terhi, Vikerpuur, Maija, Pyörälä, Satu, Atroshi, Faik |
| Zdroj: |
Microbiological Research; March 2001, Vol. 155 Issue: 4 p339-344, 6p |
| Abstrakt: |
Beta (β) and delta (δ)-hemolysin of Staphylococcus aureusstrains were cultured in vitroin milk lactoserum (whey) prepared from both healthy and mastitis bovine milk. Production of β- and δ-hemolysins were detected in 12 out of 50 strains studied. The association between N-acetyl-β-D-glucosaminidase (NAGase) activity, plasmin activity (PL) and trypsin inhibitory capacity (TIC), known as inflammatory indicators for mastitis, and hemolytic activity were also studied. Mastitic milk decreased directly the lytic effect of both β-and δ-hemolysins of S. aureuson hemolytical blood agar plates. S. aureusin healthy milk samples produced more β-hemolysin (3 times) and δ-hemolysin (2 times) when compared to S. aureussupernatants in milk from infected quarters. Furthermore, β- and b-hemolysis correlated negatively with TIC and NAGase and PL activities. Addition of reduced glutathione (GSH) or β-mercaptoethanol into the artificial medium enhanced hemolysins activity. |
| Databáze: |
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