Expression of MHC Class II Antigens on Rat Bone Marrow Cells and Macrophages, and Their Modulation during Culture with Murine GM-CSF or M-CSF

Autor: Gessl, Alois, Krugluger, Walter, Langer, Kurt, Baumgartner, Isabella, Spittler, Andreas, Grabner, Günther, Förster, Othmar, Boltz-Nitulescu, George
Zdroj: Immunobiology; February 1995, Vol. 192 Issue: 3-4 p185-197, 13p
Abstrakt: Flow cytometric analysis employing MRC OX 6 and MRC OX 17 monoclonal antibodies recognizing determinants on RT1.B or RT1.D molecules, equivalent to murine I-A and I-E, respectively, was used to detect rat MHC class II antigen (Ag) expression. Approximately 5 % of freshly isolated rat bone marrow cells (BMC) expressed RT1.B and over 30 % displayed RT1.D molecules. The RT1.D+cells were W3/13+, OX 7+, OX 19−and OX 22−. After one week culture of BMC with murine recombinant granulocyte/ macrophage colony-stimulating factor (GM-CSF), regardless of concentrations, 90 to 95 % of the cells were scored as bone marrow-derived macrophages (BMDMΦ), and over 30 % expressed both RT1.B and RT1.D Ag. GM-CSF increases the percentage of BMDMΦ bearing MHC class II Ag in a concentration-dependent manner. This effect seems to be specific because antibodies to interferon-γ, tumor necrosis factor-α or interleukin-4 did not reduce the number of cells expressing RT1.B and RT1.D Ag. Furthermore, GM-CSF was able to trigger expression of class II molecules on rat peritoneal macrophages (MΦ) and BMDMΦ resulted from cultures of BMC with mouse MΦ-CSF (M-CSF), and the RT1.B and RT1.D inducing effect of GM-CSF was opposed by M-CSF, and by anti-GM-CSF antibodies. The induction of MHC class II Ag synthesis by GM-CSF on rat BMDMΦ was confirmed at the mRNA level by Northern blot analysis employing cDNA probes encoding the RT1.Bα.
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