The changes of spectroscopic characteristics of sulfurreducing bacteria Desulfuromonas acetoxidans under the influence of different metal ions

Autor: Vasyliv, Oresta M., Bilyy, Olexandr I., Getman, Vasyl B., Kushkevych, Ivan V., Hnatush, Svitlana O.
Zdroj: Proceedings of SPIE; September 2011, Vol. 8152 Issue: 1 p81520B-81520B-7, 733688p
Abstrakt: Desulfuromonas acetoxidans, which is regarded to the oldest microorganisms that exist in the Earth, are uncoloured gram-negative obligatory anaerobic bacteria that have an ability to reduce S0to H2S. This process supports bacteria with sufficient amount of energy which they need for growth. At the same time high concentrations of hydrogen sulfide are very toxic towards all living organisms. Different metal ions that exist in surrounding environment in small concentrations are essential for microorganisms because they support normal functionality of them. But in high concentrations they have a detrimental influence on cell structure and it functions. Srains of D. acetoxidans bacteria that have high toxic metals resistance can neutralize the toxicity of hydrogen sulfide, which is the final product of dissimilative sulfurreduction, and these metals as the result of their particular binding and forming the insoluble precipitations. Light scattering changes and metals accumulation ability of D. acetoxidans bacterial cells under the influence of CuSO4, PbNO3, ZnSO4and CdSO4have been investigated. The changes of light scattering characteristics of bacterial D. acetoxidans cells on the base of their size distribution and relative content under the influence of investigated metal salts have been observed by the new method of measurement.
Databáze: Supplemental Index