Comparison of the Immunobead Binding Test (IBT) and Immunospheres (IS) Assay for Detecting Serum Antisperm Antibodies

Autor: CENTOLA, G.M., ANDOLINA, E., DEUTSCH, A.
Zdroj: American Journal of Reproductive Immunology; April 1997, Vol. 37 Issue: 4 p300-303, 4p
Abstrakt: PROBLEM: The IBT is considered the gold standard of sperm antibody assays. This test uses polyacrylamide beads labeled with antiglobulins (anti‐IgG, anti‐IgA, and anti‐IgM), which bind to the corresponding antibody on the sperm surface. The IS uses color‐coded latex beads of uniform 3.0 μm size coated with the antiglobulins which can be viewed with brightfield light microscopy. The purpose of the present study was to compare the IBT and IS in an indirect test using human serum.
Databáze: Supplemental Index