| Autor: |
Hehrlein, Christoph, Dönges, Klaus, Gollan, Christina, Metz, Jürgen, Fehsenfeld, Peter |
| Zdroj: |
Journal of the American College of Cardiology; February 1995, Vol. 25 Issue: 2, Number 2 Supplement 1 p9A-9A, 1p |
| Abstrakt: |
The long-term consequences of ionizing irradiation of arteries with doses of more than 10 Gray (Gy) are vascular stenosis. To evaluate anti-proliferative effects of much lower irradiation doses, we implanted radioactive PalmazSchatz stents (RS) emitting doses of 0.002Gy/h in rabbit arteries (n=20). The radiation dose absorbed in the arteries after 100 days is lower than a human chest dose after a single cardiac catheterization. Arterial smooth muscle cell (SMC) proliferation and neointimal hyperplasia were compared 1 and 12 weeks after RS and non-radioactive stent (NRS) implantation. SMC proliferation was assessed by immunostaining with antibodies to proliferating cell nuclear antigen (PCNA) and SMC-α-actin and by computer-assisted counting of neointimal cells (NIC)/0.1 mm2neointima. Quantitative morphometry of arterial cross-sections was applied to compare the neointimal areas after implantation of RS and NRS. After 1 week, 30% neointimal SMCs were positive for PCNA in NRS, whereas 0.5% SMCs were PCNA-positive in NRS (p< 0.01). After 12 weeks, 850±50 NIC (mean±SD) were found in arteries with NRS compared to 237±26 NIC in arteries with RS (p<0.01). The neointimal area was 0,8±0,2 mm212 weeks following implantation of NRS versus 0.4 ±0.2 mm2following RS implantation (p<0.01) |
| Databáze: |
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