| Autor: |
Sultan, Mohamed, Stecher, Gunther, Stoggl, Wolfgang, Bakry, Rania, Zaborski, P., Huck, C., El Kousy, Nagla, Bonn, Gunther |
| Zdroj: |
Current Medicinal Chemistry; March 2005, Vol. 12 Issue: 5 p573-588, 16p |
| Abstrakt: |
The article discusses the qualitative and quantitative determination of non-steroidal antiinflammatory drugs like salicin, salicylic acid, tenoxicam, ketorolac, piroxicam, tolmetin, naproxen, flurbiprofen, diclofenac and ibuprofen by reversed phase high performance liquid chromatography (RP-HPLC) and micro-HPLC (-HPLC) hyphenated with UV-absorbance and mass spectrometric detection. Both detection methods delivered calibration plots with good linearity (r2 < 0.9800), limits of detection in the low nanogram range and recovery rates between 94 and 104 . For the analysis of biological samples such as blood, plasma and erythrocytes liquid-liquid extraction (LLE) and solid phase extraction (SPE) on the basis of new synthesized glycidylmethacrylate / divinylbenzene copolymer (GMA / DVB) particles and commercially available material on the basis of poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer were investigated. Finally the use of a -HPLC system with separation columns in the range of 8 cm × 200 μm I.D. for the determination of non-steroidal anti-inflammatory drugs (NSAIDs) is presented, emphasizing on the type of column and sample amount needed. |
| Databáze: |
Supplemental Index |
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