| Autor: |
Du, Run-Pan, Jackson, Gail E. D., Wyde, Philip R., Yan, Wei-Yao, Wang, Qijun, Gisonni, Lucy, Sanhueza, Sonia E., Klein, Michel H., Ewasyshyn, Mary E. |
| Zdroj: |
Bio/Technology; August 1994, Vol. 12 Issue: 8 p813-818, 6p |
| Abstrakt: |
We have produced a genetically-engineered chimeric protein composed of the external domains of the respiratory syncytial virus (RSV) fusion (F) protein and the parainfluenza virus type 3 (PIV-3) hemagglutinin-neuraminidase (HN) protein in insect cells using the baculovirus expression system. The yield of the soluble chimeric FRSV-HNPIV-3protein could be increased approximately 2-fold by using Trichoplasia ni(High Five) insect cells in place of Spodoptera frugiperda(Sf9) for expression. The chimeric protein, purified from the supernatant of baculovirus-infected High Five cells by immunoaffinity chromatography was correctly processed at the F2-F1 proteolytic cleavage site. Immunochemical analysis of the chimera with a panel of anti-F and anti-HN monoclonal antibodies suggested that the antigenicity of the major F and HN neutralization epitopes of the chimeric protein was preserved. Immunization of cotton rats with two 1 or 10 μg doses of the chimeric protein adsorbed to aluminum phosphate elicited strong PIV-3 specific HAI responses as well as PIV-3 and RSV specific neutralizing antibodies, and at either dose completely protected against challenge with live RSV and PIV-3. |
| Databáze: |
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