| Autor: |
Nyyssönen, Eini, Penttilä, Merja, Harkki, Anu, Saloheimo, Anu, Knowles, Jonathan K. C., Keränen, Sirkka |
| Zdroj: |
Bio/Technology; May 1993, Vol. 11 Issue: 5 p591-595, 5p |
| Abstrakt: |
We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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