| Autor: |
Weaver, James C., Bliss, Jonathan G., Powell, Kevin T., Harrison, Gail I., Williams, Gregory B. |
| Zdroj: |
Bio/Technology; September 1991, Vol. 9 Issue: 9 p873-877, 5p |
| Abstrakt: |
We describe a new, general method for rapidly measuring clonal growth of large numbers of individual members of a cell population. This method is based on microculture of individual colony-forming units in gel microdrops (GMDs; here aga-rose; 20 to 90μ in diameter), which are sufficiently robust to be handled much like cells, and diffusionally transparent for molecules of interest. Flow cytometry provides rapid measurements of GMD-entrapped microcolonies, and permits sub-population analysis. Here the method is demonstrated with mammalian, fungal and bacterial species. Additional results illustrate rapid determination of a drug-resistant subpopulation in a mixed species sample, and nutrient sensitivity for a murine hybridoma. |
| Databáze: |
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