Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus Nidulans

Autor: Upshall, A., Kumar, A. A., Bailey, M. C., Parker, M. D., Favreau, M. A., Lewison, K. P., Joseph, M. L., Maraganore, J. M., McKnight, G. L.
Zdroj: Bio/Technology; December 1987, Vol. 5 Issue: 12 p1301-1304, 4p
Abstrakt: Strains of the filamentous fungus Aspergillus nidulans transformed with a functional cDNA comprising the entire coding region of human tissue plasminogen activator (t-PA) secrete biologically active protein into the culture medium. Activity is inhibited by treatment with t-PA specific polyclonal antibodies. The protein is correctly processed at the amino terminus and is not excessively glycosylated.
Databáze: Supplemental Index