Light-Inducible Expression of a Chimeric Gene in Soybean Tissue Transformed with Agrobacterium

Autor: Facciotti, D., O'Neal, J. K., Lee, S., Shewmaker, C. K.
Zdroj: Bio/Technology; March 1985, Vol. 3 Issue: 3 p241-246, 6p
Abstrakt: Soybean (Glycine max cv. “Forrest”) has been successfully transformed with Agrobacterium containing a bacterial kanamycin resistance gene linked to the 5′ portion of a soybean small subunit carboxylase gene. Comparison of light-grown versus dark-grown transformed callus demonstrated light induction of the chimeric gene as measured by increased levels of kanamycin resistance. This is not unexpected as small subunit genes are under light regulation.
Databáze: Supplemental Index