| Autor: |
Wallace, Bruce G., Nitkin, Ralph M., Reist, Noreen E., Fallon, Justin R., Moayeri, N. Nicole, McMahan, U. J. |
| Zdroj: |
Nature; June 1985, Vol. 315 Issue: 6020 p574-577, 4p |
| Abstrakt: |
Basal lamina-rich extracts of Torpedo californica electric organ contain a factor that causes acetylcholine receptors (AChRs) on cultured myotubes to aggregate into patches1–3. Our previous studies have indicated that the active component of these extracts is similar to the molecules in the basal lamina which direct the aggregation of AChRs in the muscle fibre plasma membrane at regenerating neuromuscular junctions in vivo2,4,5. Because it can be obtained in large amounts and assayed in controlled conditions in cell culture, the AChR-aggregating factor from electric organ may be especially useful for examining in detail how the postsynaptic apparatus of regenerating muscle is assembled. Here we demonstrate that the electric organ factor causes not only the formation of AChR aggregates on cultured myotubes, but also the formation of patches of acetylcholinesterase (AChE). This finding, together with the observation that basal lamina directs the formation of both AChR and AChE aggregates at regenerating neuromuscular junctions in vivo6, leads us to hypothesize that a single component of the synaptic basal lamina causes the formation of both these synaptic specializations on regenerating myofibres. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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