| Autor: |
Hardy, R. R., Hayakawa, K., Parks, D. R., Herzenberg, L. A. |
| Zdroj: |
Nature; November 1983, Vol. 306 Issue: 5940 p270-272, 3p |
| Abstrakt: |
CBA/N mice carrying the X-linked immune deficiency gene (xid) have fewer splenic B cells than normal CBA mice and are unresponsive to a certain class of antigens1. Studies of B-cell surface-marker expression2and immune responsiveness3have led to the commonly accepted idea that the B cells in adult xid mice are immature and resemble the B cells of young (1–3 week old) normal mice. That is, like young animals, xid mice lack cells in the most numerous of three IgM/IgD B-cell sub-populations (designated I in Fig. 1a, b) present in adult spleen4,5. We now report, however, that this picture is an oversimplification and that in fact the B cells in adult xid mice differ from those present in either adult or young normal mice. Using quantitative three-colour fluorescence-activated cell sorter (FACS) analyses, we have compared the correlated expression of IgM, IgD and a newly discovered B-lymphocyte antigen (BLA-1) on splenic B cells in normal and xid mice. We show here (1) that most B cells in adult xid mice (as in normals) are BLA-1−whereas all B cells in young animals are BLA-1+; (2) that the major difference in the IgM/IgD B-cell subpopulations found between xid and normal mice is limited to the BLA-1−cells; and (3) that xid mice have increased numbers of BLA-1+population III B cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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