| Autor: |
WERRLEIN, ROBERT J., GLINOS, ANDRÉ D. |
| Zdroj: |
Nature; September 1974, Vol. 251 Issue: 5473 p317-319, 3p |
| Abstrakt: |
OXYGEN transport in cultures of attached mammalian cells depends on the kinetics of gas diffusion through the overlaying medium. Working with liver parenchymal cells, Stevens1calculated that the oxygen requirements and energy demands of a monolayer culture could not be met by atmospheric pO2if the fluid overlay was deeper than 0.34 mm. McLimans2theorised that under the standard medium overlay of 1.5–5.0 mm, oxygen depletion by a respiring monolayer of liver parenchymal cells would exhaust the atmospheric oxygen supply at the plane of the attached cell sheet within 35 min. His model suggests that adaptive growth of cultured cells occurs only when respiration does not exceed the delivery of oxygen to the monolayer. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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