| Autor: |
WARDELL, W. M., TOMITA, T. |
| Zdroj: |
Nature; December 1967, Vol. 216 Issue: 5119 p1007-1008, 2p |
| Abstrakt: |
THE double barrelled microelectrode1is a convenient way of introducing two electrode tips into the same cell for simultaneously passing a current and recording. The chief source of artefact is the coupling resistance (Rc) (ref. 2), which is manifest when a current passed through the stimulating barrel causes a voltage to appear at the recording barrel. It presumably represents the resistance of electrolyte immediately adjacent to and shared by both tips, for most of it disappears if the very tip of the electrode is broken off. When current is passed from an intracellular double barrelled electrode, the coupling resistance with the electrode intracellular (Rci) is in series with the cell membrane resistance viewed by the recording barrel. Rcimust therefore be subtracted from the observed input resistance to obtain the true value of the input resistance and of voltage displacements. The problem is to know the value of Rci, because Rcican only be measured directly with the electrode extracellular. The simplest method2is to assume that Rcidoes not change on impalement, which is to assume that Rciis the same as Rci—the value obtained on withdrawal from the same cell. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
