| Autor: |
Nolden, Lars, Beckers, Gabriele, Möckel, Bettina, Pfefferle, Walter, Nampoothiri, K.Madhavan, Krämer, Reinhard, Burkovski, Andreas |
| Zdroj: |
FEMS Microbiology Letters; August 2000, Vol. 189 Issue: 2 p305-310, 6p |
| Abstrakt: |
The Corynebacterium glutamicumgenes encoding urease were isolated and sequenced. While ureA, ureBand ureCare encoding structural subunits of urease, ureE, ureF, ureGand ureDare encoding accessory proteins. As deduced from DNA sequence analyses, the uregenes are transcriptionally coupled, this was proven by RT‐PCR at least for ureABC. Gene disruption experiments revealed that both structural (UreC) and accessory proteins (UreD) are indispensable for urease activity and growth on urea. Urease activity was determined in different Corynebacteriumspecies after growth in various media. While the regulation patterns observed revealed species‐specific differences, in general urease activity is induced upon nitrogen starvation. As in mycobacteria, in corynebacteria urease activity was highest in a pathogenic species and might also play a role in host–pathogen interaction. |
| Databáze: |
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