| Autor: |
Stubbs, Simon, Rupnik, Maja, Gibert, Maryse, Brazier, Jon, Duerden, Brian, Popoff, Michel |
| Zdroj: |
FEMS Microbiology Letters; May 2000, Vol. 186 Issue: 2 p307-312, 6p |
| Abstrakt: |
In addition to the two large clostridial cytotoxins (TcdA and TcdB) certain strains of Clostridium difficileproduce an actin‐specific ADP‐ribosyltransferase, or binary toxin. PCR reactions were developed to detect genes encoding the enzymatic (cdtA) and binding (cdtB) components of the binary toxin and 170 representative strains were tested to assess the prevalence of the toxin. Positive PCR results (n=59) were confirmed by immunoblotting and ADP‐ribosyltransferase assay. PCR ribotype and toxinotype (restriction fragment length polymorphism analysis of genes for TcdA and TcdB) correlated with possession of binary toxin genes. All strains with cdtAand cdtBbelonged to toxin‐variable toxinotypes and five toxin‐producing groups of strains have been described according to the presence or absence of TcdA, TcdB and binary toxin. Result indicate that ca. 6.4% of toxigenic isolates of C. difficilereferred to the Anaerobe Reference Unit from UK hospitals have cdtAand cdtBgenes. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Plný text