| Autor: |
Spreng, Simone, Gentschev, Ivaylo, Goebel, Werner, Mollenkopf, Hans‐Joachim, Eck, Mathias, Müller‐Hermelink, Hans Konrad, Schmausser, Bernd |
| Zdroj: |
FEMS Microbiology Letters; May 2000, Vol. 186 Issue: 2 p251-256, 6p |
| Abstrakt: |
We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylorivia the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pyloriprotein (or protein domain) is inserted in‐frame into a residual portion of the hemolysin gene (hlyA), encoding the HlyA secretion signal (HlyAs). These fusion proteins are secreted efficiently by E. coli. This new approach allows the identification of immunodominant antigens by using sera derived from H. pylori‐infected patients suffering from different gastroduodenal pathologies. Three immunodominant antigens bearing the ureB(urease B‐subunit), flaA(flagellin A‐subunit), and an unknown ORF (HP0888) encoding an E. coliFecE analogous protein fused to hlyAswere identified and characterized. |
| Databáze: |
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