Biodegradation of p‐toluenesulphonamide by a Pseudomonassp.

Autor: Haperen, A.M., Velde, J.W., Ginkel, C.G.
Zdroj: FEMS Microbiology Letters; November 2001, Vol. 204 Issue: 2 p299-304, 6p
Abstrakt: A bacterium capable of utilising p‐toluenesulphonamide was isolated from activated sludge. The isolated strain designated PTSA was identified as a Pseudomonassp. using chemotaxonomic and genetic studies. PseudomonasPTSA grew on p‐toluenesulphonamide in a chemostat with approximately 90% release of sulphate and 80% release of ammonium. The isolate was also able to grow on 4‐carboxybenzenesulphonamide and 3,4‐dihydroxybenzoate but did not grow on p‐toluenesulphonate. The transient appearance of 4‐hydroxymethylbenzenesulphonamide and 4‐carboxybenzenesulphonamide during p‐toluenesulphonamide degradation proves oxidation of the methyl group is the initial attack in the biodegradation pathway. Both metabolites of p‐toluenesulphonamide degradation were identified by high‐performance liquid chromatography‐mass spectrometry. 4‐Carboxybenzenesulphonamide is probably converted into 3,4‐dihydroxybenzoate and amidosulphurous acid. The latter is a chemically unstable compound in aqueous solutions and immediately converted into sulphite and ammonium. Both sulphite and ammonium were formed during degradation of 4‐carboxybenzenesulphonamide.
Databáze: Supplemental Index