| Autor: |
Licht, Tine Rask, Hammerum, Anette M, Jensen, Lars Bog?, Jacobsen, Bodil L |
| Zdroj: |
FEMS Microbiology Letters; November 2001, Vol. 204 Issue: 2 p305-309, 5p |
| Abstrakt: |
The effect of synthetic sex pheromone on pheromone‐inducible conjugation between the isogenic Enterococcus faecalisstrains OG1RF and OG1SS was investigated in (i) Todd‐Hewitt broth medium and (ii) intestinal mucus isolated from germ‐free rats. In broth, the presence of synthetic pheromone cCF10 had no detectable effect on the transfer kinetics observed for the tetracycline resistance encoding plasmid pCF10. In mucus, presence of the same pheromone significantly increased the transfer efficiency observed during the first 2 h of conjugation, while the effect was less pronounced later in the experiment. We suggest that due to differences in diffusion rates and medium‐binding of the pheromones, the effect of the synthetic cCF10 was immediately dominated by the effect of pheromones produced by the recipient E. faecalisstrain in broth, while this happened later in mucus. |
| Databáze: |
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