Characterisation of Listeria ivanoviiisolates from the UK using pulsed‐field gel electrophoresis

Autor: Ramage, Clifford P, Low, J.Christopher, McLauchlin, James, Donachie, William
Zdroj: FEMS Microbiology Letters; January 1999, Vol. 170 Issue: 2 p349-353, 5p
Abstrakt: Forty‐three Listeria ivanoviiisolates were collected in the UK between 1991 and 1997 from: 35 animal infections; two human infections; five foods; and one environmental source. A further two type strains of L. ivanovii(subsp. ivanoviiand subsp. londoniensis) were obtained from a culture collection. These bacteria were characterised by conventional phenotypic methods and by pulsed‐field gel electrophoresis (PFGE) using ApaI and SmaI. Forty‐two of the isolates from the UK were identified as L. ivanoviisubsp. ivanoviiand the remaining culture as L. ivanoviisubsp. londoniensis. Six and four PFGE profiles were obtained using ApaI and SmaI digestion respectively; six composite profiles were obtained combining the results for both enzymes. The PFGE profile of the UK L. ivanoviisubsp. londoniensis(isolated from processed shrimps) was similar to the type strain of this subspecies and differed from all of the L. ivanoviisubsp. ivanoviitested. The majority of isolates (38 out of 45) belonged to one profile showing that the UK population of this bacterium is much less genetically diverse than similar studies have shown for Listeria monocytogenes.
Databáze: Supplemental Index