| Autor: |
Tschierske, M, Ehlert, K, Strandén, A.M, Berger‐Bächi, B |
| Zdroj: |
FEMS Microbiology Letters; August 1997, Vol. 153 Issue: 2 p261-264, 4p |
| Abstrakt: |
The formation of the Staphylococcus aureuspeptidoglycan pentaglycine interpeptide chain needs FemA and FemB for the incorporation of glycines Gly2‐Gly3, and Gly4‐Gly5, respectively. The lysostaphin immunity factor Lif was able to complement FemB, as could be shown by serine incorporation and by an increase in lysostaphin resistance in the wild‐type as well as in a femBmutant. However, Lif could not substitute for FemA in femAor in femAB‐null mutants. Methicillin resistance, which is dependent on functional FemA and FemB, was not complemented by Lif, suggesting that serine‐substituted side chains are a lesser substrate for penicillin‐binding protein PBP2′ in methicillin resistance. |
| Databáze: |
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