| Autor: |
Lonergan, Greg, Mew, Elizabeth, Schliephake, Kirsten, Baker, Warren L |
| Zdroj: |
FEMS Microbiology Letters; August 1997, Vol. 153 Issue: 2 p485-490, 6p |
| Abstrakt: |
A fluorometric procedure has been developed for detection and estimation of laccase activity in fungal broth cultures. Laccase solution was pretreated with catalase for 1 h at 37°C and pH 5. Homovanillic acid was then added and the reaction mixture incubated for a further hour at 37°C. The fluorescence was then developed by addition of 0.1 M glycine buffer at pH 10. Laccase preparations from Pyricularia oryzae, Coriolus hirsutusand Pycnoporus cinnabarinuscatalysed formation of a fluorescent product of HVA but the optimum pH values of enzyme activities varied. The culture fluids of several other fungi also catalysed development of fluorescence in solutions containing HVA. p‐Hydroxyphenylacetic acid was a poor substrate for all laccases in vivo except that produced by Perennipora tephropora. |
| Databáze: |
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