| Autor: |
Seishima, Mariko, Nojiri, Mari, Akiyama, Tomoko, Seishima, Mitsuru, Noma, Akio, Etoh, Yuzuru, Kitajima, Yasuo |
| Zdroj: |
FEBS Letters; January 1996, Vol. 398 Issue: 1 p120-124, 5p |
| Abstrakt: |
Activins are members of the TGF-β superfamily and are classified into 3 types: activin A, which consists of a homodimer of βA, activin B, which consists of a homodimer of βB, and activin AB, which consists of a heterodimer of βAβB. We studied the expression of activin mRNAs by RT-PCR in normal human epidermis, cultured keratinocytes, and DJM-1 cells (a squamous cell carcinoma line). We could detect only activin A mRNA ( βA) in normal human epidermis. In cultured keratinocyte and DJM-1 cells, activin βAmRNA was observed at 4 h but not at 96 h after plating. Activin A activity was detected in the conditioned medium of DJM-1 cells within 48 h. In addition, although follistatin mRNA was not observed in human epidermis in situ, it was transiently expressed in cultured cells at 4 h after plating. These findings suggest that the expression of these molecules in keratinocytes is associated with cell proliferation. In an in vitro tissue injury model, activin A was observed at the wound edge, where cell migration and proliferation may be activated. In DJM-1 cells cultured for 92 h, βAmRNA was observed 4 h after injury treatment. These findings suggest that activin A acts as a potent inducer of proliferation in vitro, at least in keratinocytes. |
| Databáze: |
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