| Autor: |
Luca, Nicola G, Wexler, Margaret, Pereira, Maria J, Yeoman, Kay H, Johnston, Andrew W.B |
| Zdroj: |
FEMS Microbiology Letters; November 1998, Vol. 168 Issue: 2 p289-295, 7p |
| Abstrakt: |
Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarumbiovar viciae, the nitrogen‐fixing symbiont of peas, was isolated and sequenced. The furgene is normally expressed constitutively, independent of the presence of Fe in the medium, but in one Rhizobiumstrain it was transcribed at a low level. Attempts to isolate a furknockout mutant failed, suggesting that the gene is essential for free‐living growth. In other bacteria, certain furmutations confer manganese resistance; however, none of the manganese‐resistant mutants of R. leguminosarumwhich we isolated was corrected by the cloned furgene. When the cloned R. leguminosarum furgene was introduced into a furmutant of Escherichia coli, it caused some Fe‐dependent reduction in the amount of siderophore, indicating that it can function heterologously. |
| Databáze: |
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