| Autor: |
Springer, Amy L, Auman, Ann J, Lidstrom, Mary E |
| Zdroj: |
FEMS Microbiology Letters; March 1998, Vol. 160 Issue: 1 p119-124, 6p |
| Abstrakt: |
In the facultative serine cycle methylotroph Methylobacterium extorquensAM1, mxaBis required for regulation of methanol oxidation and is located at the end of a large cluster of methylotrophy genes that begins with mxaF. The sequence of mxaBhas been obtained and indicates that the gene product is a member of the response regulator family. None of the open reading frames near mxaBshowed sequence identity to sensor kinases. Complementation studies suggest a promoter may be located adjacent to mxaB. Another gene (mxaW) is present immediately upstream of mxaF, divergently transcribed from a methanol‐inducible promoter. The sequence in the region of mxaWwas also obtained. MxaWshowed no identity to known proteins. Mutations in mxaWand in an adjacent open reading frame, OrfR, had no effect on growth of M. extorquensAM1 on methanol or other substrates. The MxaW mutant had normal methanol dehydrogenase activity and normal transcription of the mxaFpromoter. Therefore, the function of mxaWis unknown. |
| Databáze: |
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