| Autor: |
Maitra, P.K., Bhosale, S.B., Kshirsagar, Deepa C, Yeole, T.Y., Shanbhag, Archana N |
| Zdroj: |
FEMS Microbiology Letters; April 2001, Vol. 198 Issue: 1 p23-29, 7p |
| Abstrakt: |
When a buffered anaerobic cell suspension of Methanococcoides methylutenswas maintained under methanol‐limited conditions, intracellular glycogen and hexose phosphates were consumed rapidly and a very small amount of methane formed at 4 h of a starvation period. When methanol was supplemented after a total of 20 h of starvation, a reverse pattern was observed: the glycogen level and the hexose phosphate pool increased, and formation of methane took place after a lag period of 90 min. A considerable amount of methane was formed in 120 min after its detection with a rate of 0.18 μmol mg−1protein min−1. When methane formation decreased after 270 min of incubation and finally came to a halt, probably due to complete assimilation of supplemented methanol, the levels of glycogen and hexose monophosphates decreased once again. However fructose 1,6‐diphosphate levels showed a continuous increase even after exhaustion of methane formation. In contrast to the hexose phosphate pool, levels of other metabolites showed a small increase after addition of methanol. The enzyme profile of glycogen metabolism showed relatively high levels of triose phosphate isomerase. Glyceraldehyde 3‐phosphate dehydrogenase reacted with NADPH with a three‐fold higher activity as compared to that with NADH. |
| Databáze: |
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