Osmolarity modulates the expression of the Hha protein from Escherichia coli

Autor: Mouriño, Mercedes, Balsalobre, Carlos, Madrid, Cristina, Nieto, José Marıća, Prenafeta, Antoni, Muñoa, Francisco J, Juárez, Antonio
Zdroj: FEMS Microbiology Letters; March 1998, Vol. 160 Issue: 2 p225-229, 5p
Abstrakt: The effect of the osmolarity of the culture medium on the expression of the hhagene of Escherichia coliwas investigated. When cells were grown in LB medium, expression reached a maximum in the exponential phase of growth and decreased in the stationary phase. Increasing the osmolarity of the LB medium had no significant effect on the expression of the hhagene, but depletion of NaCl led to a significant decrease in expression. Expression of the hhagene is thus sensitive to the osmolarity of the growth medium. High levels of expression of the hhagene when cells are grown at medium to high osmolarity are consistent with the finding that the Hha protein appears to play its main modulatory role when cells grow under these conditions.
Databáze: Supplemental Index