| Autor: |
Berto, Philippe, Comménil, Pascal, Belingheri, Lionel, Dehorter, Bertrand |
| Zdroj: |
FEMS Microbiology Letters; November 1999, Vol. 180 Issue: 2 p183-189, 7p |
| Abstrakt: |
Alternaria brassicicolais a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80‐kDa lipase (E.C 3.1.1.3) was detected by SDS‐PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross‐reacted with Botrytis cinereaanti‐lipase antibodies, which were reported to inhibit the in vitro lipase activity. Anti‐lipase antibodies were added to a conidial suspension of A. brassicicolaprior to inoculation. As a result, blackspot lesions were reduced by 90% on intact cauliflower leaves, but not on leaves from which surface wax had been removed. Spore surface‐bound lipase is thought to interact closely with epicuticular leaf waxes for adhesion and/or penetration of the fungal propagules during the early stages of host‐parasite interactions. |
| Databáze: |
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