| Autor: |
Baida, Gleb, Budarina, Zhanna I, Kuzmin, Nikolai P, Solonin, Alexander S |
| Zdroj: |
FEMS Microbiology Letters; November 1999, Vol. 180 Issue: 1 p7-14, 8p |
| Abstrakt: |
Hemolysin II gene from Bacillus cereusVKM‐B771 has been sequenced. The deduced primary translation product consists of 412 amino acid residues which corresponds to the protein with an Mrof 45.6 kDa. The predicted mature Hly‐II protein (residues 32 to 412) is of 42.3 kDa, which is in close agreement with the mini‐cell electrophoresis analysis. Hly‐II deletion variant lacking 96 C‐terminal residues still has hemolytic activity. The protein primary structure analysis revealed no homology with any known Bacilluscytolysins. Significant general homology (31–28% identity) was found between the hemolysin II and Staphylococcus aureusalpha‐toxin, gamma‐hemolysin (HlgB), and leukocidins (LukF, LukF‐R, LukF‐PV). The data suggest that hemolysin II belongs to the group of β‐channel forming cytolysins. |
| Databáze: |
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