| Abstrakt: |
The electrophoretic karyotype of the filamentous fungus Penicillium purpurogenumhas been resolved. Using contour‐clamped homogeneous electric field gel electrophoresis, five chromosomal bands were separated, with estimated sizes of 7.1, 5.2, 3.7, 2.9 and 2.3 Mbp, giving a total genome size of 21.2 Mbp. To our knowledge, this is the smallest Penicilliumgenome determined so far. By Southern blots and using homologous probes, the chromosomal location of five xylanolytic genes from P. purpurogenumwas determined: axeI(acetyl xylan esterase I), xynB(endoxylanase B) and abf1(arabinofuranosidase 1) in chromosome I, xynA(endoxylanase A) in chromosome II, and axeII(acetyl xylan esterase II) in chromosome III. This is the first study where the location of xylanase genes in a Penicilliumgenome has been established. |
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