Comparison of eae, tir, espAand espBgenes of bovine and human attaching and effacing Escherichia coliby multiplex polymerase chain reaction

Autor: China, Bernard, Goffaux, Frédéric, Pirson, Vinciane, Mainil, Jacques
Zdroj: FEMS Microbiology Letters; September 1999, Vol. 178 Issue: 1 p177-182, 6p
Abstrakt: Attaching and effacing Escherichia coli(AEEC) virulence genes include the eae, the tir, the espAand the espBgenes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype.
Databáze: Supplemental Index