| Autor: |
Ikigai, Hajime, Ono, Toshihisa, Iwata, Masashi, Nakae, Taiji, Shimamura, Tadakatsu |
| Zdroj: |
FEMS Microbiology Letters; May 1997, Vol. 150 Issue: 2 p249-254, 6p |
| Abstrakt: |
We investigated the channel formation by El Tor hemolysin (molecular mass, 65 kDa) of Vibrio choleraeO1 biotype El Tor in planar lipid bilayers. The El Tor hemolysin channel exhibited asymmetric and hyperbolic membrane current with increasing membrane potential, meaning that the channel is voltage dependent. The zero‐current membrane potential measured in KCl solution showed that permeability ratio PK+/PCl−was 0.16, indicating that the channel is 6‐fold more anion selective over cation. The hemolysin channel frequently flickered in the presence of divalent cations, suggesting that the channel spontaneously opens and closes. These data imply that the El Tor hemolysin damages target cells by the formation of transmembrane channels and, consequently, is the cause of osmotic cytolysis. |
| Databáze: |
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