| Autor: |
Spano, Furio, Putignani, Lorenza, McLauchlin, James, Casemore, David P, Crisanti, Andrea |
| Zdroj: |
FEMS Microbiology Letters; May 1997, Vol. 150 Issue: 2 p209-217, 9p |
| Abstrakt: |
Cryptosporidium wrairiwas isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairidisplayed a different host range and site of infection and may represent a separate species or sub‐species. We used the polymerase chain reaction to clone two distinct 550 bp‐long DNA fragments, Wc‐I and Wc‐II, of the gene encoding the Cryptosporidiumoocyst wall protein (COWP) of C. wrairi,which showed 98% identity to the C. parvumhomologue. Within Wc‐I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction‐restriction fragment length polymorphism method able to distinguish C. wrairifrom C. parvumand to identify two groups of C. parvumisolates differentially associated with animal and human infections. |
| Databáze: |
Supplemental Index |
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