| Autor: |
Chen, Baowei, Menon, Nanda K., Dervertarnian, Lisa, Moura, Jose J.G., Przybyla, Alan E. |
| Zdroj: |
FEBS Letters; January 1994, Vol. 351 Issue: 3 p401-404, 4p |
| Abstrakt: |
We have cloned the gene encoding Desulfovibrio gigasferredoxin using a photodigoxigenin-labelled probe synthesized with the polymerase chain reaction. The DNA sequence of the gene predicts a polypeptide of 58 residues after removal of the initial formyl methionine (polypeptide Mr= 6,276). The ferredoxin gene was expressed in aerobically grown E. colibehind the lacpromoter of pUC18 resulting in a high level of ferredoxin expression which comprises about 10% of the total cell protein. EPR analysis of recombinant ferredoxin revealed the presence of a [3Fe-4S] cluster which is characteristic of native D. gigasferredoxin II. |
| Databáze: |
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