| Autor: |
Siegmund, Klaus-Detlef, Klink, Friedrich |
| Zdroj: |
FEBS Letters; January 1992, Vol. 312 Issue: 2 p139-142, 4p |
| Abstrakt: |
An antiserum to ADP-ribosylated elongation factor 2 (ADPR-EF-2) from S. acidocaldariuswas raised in rabbits using stained, homogenized, ADPR-EF-2-containing slices from SDS-gels as a source of antigen. Elongation factor 2 (EF-2) from S. acidocaldariuswas cloned in E. coliand the expressed gene product was used in order to adsorb all anti-EF-2 antibodies which do not contain the ADP-ribosyl group within their epitopes, as E. coliis unable to synthesize the ADP-ribosyl acceptor diphthamide. The remaining antibodies were specific to ADP-ribosylated EF-2 from Thermoplasma acidophilum, S. acidocaldariusand Desulfurococcus mucosus. ADP-ribosylated EF-2 from eukaryotic sources also reacted with the adsorbed antiserum as shown for EF-2 isolated from the killi-fish Cynolebias whitei, the mouse species BALB/c and Han/Wistar rats. The adsorbed antiserum did not cross-react with ADP-ribosylated actin or rho protein or with FAD-containing D-amino acid oxidase. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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