Evaluation of the use of microemulsions as eluents in high‐performance liquid chromatography

Autor: El‐Sherbiny, Dina Tawfik Mohamed, El‐Ashry, Saadia Mohamed, Mustafa, Mohamed Ahmed, Abd‐El‐Rahman El‐Emam, Ali, Hansen, Steen Honoré
Zdroj: Journal of Separation Science (JSS) (formerly Journal of High Resolution Chromatography); May 2003, Vol. 26 Issue: 7 p503-509, 7p
Abstrakt: An overview of the use of microemulsions as eluents in HPLC is given. The separation mechanisms involved are discussed and the technique is compared to solvent modified micellar chromatography as well as to ordinary reversed‐phase HPLC in order to evaluate differences and similarities. The microemulsion systems have been used for the separation of a number of antihypertensives and non steroidal antiinflammatory drug substances as well as for the analysis of bumetanide and acebutolol in plasma and urine. The plasma samples may be injected directly onto the HPLC system after a simple dilution.
Databáze: Supplemental Index